БИОТЕХНОЛОГИЯ ЛАКТУЛОЗЫ: СОВРЕМЕННЫЕ ДОСТИЖЕНИЯ, ПРОБЛЕМЫ И ПЕРСПЕКТИВЫ
Аннотация и ключевые слова
Аннотация (русский):
Лактулоза является пребиотиком, который широко применяется в медицине и пищевой промышленности. Производство лактулозы основано на изомеризации лактозы в щелочных средах при высоких температурах. Альтернативой являются ферментативные способы, которые считаются более экологически чистыми и позволяют проводить процессы в более мягких условиях. Объектом исследования стали научные публикации по вопросам синтеза и очистки лактулозы с использованием ферментов. Для поиска информации были использованы базы данных Scopus, Web of Science, PubMed и Elibrary за период с 1978 по 2022 гг. Рассмотрели два основных пути ферментативного превращения лактозы в лактулозу: изомеризация (прямой) и трансгалактозилирование (с промежуточным гидролизом). Для первого применяют целлюлозо-2-эпимеразы, которые пока не имеют статуса безопасности и их не производят в промышленных масштабах, а побочным продуктом их реакции является эпилактоза. Для трансгалактозилирования применяют β-галактозидазы, которые имеют международный статус безопасности (GRAS) и доступны на рынке, с хорошо изученным механизмом действия. Систематизировали данные об условиях получения максимальных выходов лактулозы при использовании разных ферментов. Основными продуцентами β-галактозидаз для получения лактулозы являются дрожжи Kluyveromyces lactis и плесени Aspergillus оryzae. Выход лактулозы достигает 30 % в оптимальных условиях. Основной проблемой является необходимость внесения фруктозы. Прямой зависимости между максимальными выходами лактулозы и молярными соотношениями фруктоза:лактоза не выявлено. Применение целлобиозо-эпимераз позволяет достигать высоких выходов лактулозы (70–80 %), но для получения этих ферментов используют методы генной инженерии и мутагенеза, что ставит под сомнение их безопасность. К наиболее перспективным тенденциям развития биотехнологии лактулозы можно отнести использование вторичного молочного сырья и иммобилизованных ферментов, применение разных моделей реакторов, в т. ч. мембранных, и разработку комплексных взаимосвязанных процессов получения ферментов, конверсии лактозы в лактулозу и очистки готовых продуктов.

Ключевые слова:
Лактулоза, лактоза, биоконверсия, ферменты, выход, β-галактозидаза, Kluyveromyces, Aspergillus, целлобиозо-2-эпимераза, тенденции
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