Abstract and keywords
Abstract (English):
A more efficient bioconversion of renewable plant resources is a priority in modern biotechnology. An important aspect of the processing and pretreatment of cellulose raw materials is to obtain a high content of reducing substances in the final product. The present research objective was to determine the optimal conditions for the chemical transformation of plant polymers to obtain biologically valuable substances. The research results will reduce the final cost of biotechnological production. This research featured wheat bran polymers treated with sulfuric acid and relied on a set of standard research methods. The degree of polymer conversion was tested on native and mechanically activated wheat bran fractions of 600, 200, and 100 microns. The kinetics of the high-temperature chemical hydrolysis was as follows: temperature – 120–130°C, sulfuric acid concentration – 0.6–0.9%, treatment time – 30–60 min, hydromodule – 1:8;9;10. The quantitative and qualitative composition of mono- and disaccharides of hydrolysates was determined using the high performance liquid chromatography method. The composition of wheat bran showed a low content of lignin (7.55%) and a high content of pentosans (17.9%). The highest content of reducing substances in hydrolysates was 640 mg/g bran. The optimal technological conditions with the highest content of reducing substances were as follows: hydromodulus – 1:10, temperature – 120°C, treatment time – 45 min, and sulfuric acid concentration – 0.9%. The greatest change in the content of mono- and disaccharides of hydrolysates belonged to pentoses: 78.2 mg/g of bran (in terms of xylose). The amount of easily hydrolysable carbohydrates and wheat bran fiber decreased by 80 and 19%, respectively. This research revealed the optimal parameters for the chemical hydrolysis of wheat bran to obtain biologically valuable carbohydrates. This area of research can be of practical use for producers of biofuels, chemicals, and food additives.

Wheat bran, chemical hydrolysis, carbohydrate-containing raw materials, chromatography, mechanical activation
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1. Clifton-Brown J, Harfouche A, Casler MD, Jones HD, Macalpine WJ, Murphy-Bokern D, et al. Breeding progress and preparedness for mass-scale deployment of perennial lignocellulosic biomass crops switchgrass, miscanthus, willow and poplar. GCB Bioenergy. 2018;11(1):118–151.

2. Wang L, Tian Y, Chen Y, Chen J. Effects of acid treatment on the physicochemical and functional properties of wheat bran insoluble dietary fiber. Cereal Chemistry. 2022;99(2):343–354.

3. Jia M, Chen J, Liu X, Xie M, Nie S, Yi C, et al. Structural characteristics and functional properties of soluble dietary fiber from defatted rice bran obtained through Trichoderma viride fermentation. Food Hydrocolloids. 2019;94:468–474.

4. Bhatia L, Sharma A, Bachheti RK, Chandel AK. Lignocellulose derived functional oligosaccharides: production, properties, and health benefits. Preparative Biochemistry and Biotechnology. 2019;49(8):744–758.

5. Awasthi MK, Tarafdar A, Gaur VK, Amulya K, Narisetty V, Yadav DK, et al. Emerging trends of microbial technology for the production of oligosaccharides from biowaste and their potential application as prebiotic. International Journal of Food Microbiology. 2022;368.

6. Bhatia R, Lad JB, Bosch M, Bryant DN, Leak D, Hallett JP, et al. Production of oligosaccharides and biofuels from Miscanthus using combinatorial steam explosion and ionic liquid pretreatment. Bioresource Technology. 2021;323.

7. Procentese A, Raganati F, Olivieri G, Russo ME, Rehmann L, Marzocchella A. Deep Eutectic Solvents pretreatment of agro-industrial food waste. Biotechnology for Biofuels and Bioproducts. 2018;11.

8. Wen Y, Niu M, Zhang B, Zhao S, Xiong S. Structural characteristics and functional properties of rice bran dietary fiber modified by enzymatic and enzyme-micronization treatments. LWT. 2017;75:344–351.

9. Bashashkina EV, Souyasov NA, Shakir IV, Panfilov VI. Bioconversion of soluble coffee production waste into fodder products. Ecology and Industry of Russia. 2011;(1):18–19. (In Russ.).

10. Smirnova VD, Kisseleva RYu, Shakir IV, Panfilov VI. A biotechnological way of processing of waste products of soya-protein manufacture. Ecology and Industry of Russia. 2010;(5):14–16. (In Russ.).

11. Vasilʹev AV, Panfilov VI, Shakir IV, Afanaʹsv AV, Tsygankov MA. Acid and enzymatic hydrolysis of waste from the brewing. Chemical Technology. 2007;8(1):17–21. (In Russ.).

12. Taherzadeh MJ, Karimi K. Acid-based hydrolysis processes for ethanol from lignocellulosic materials: a review. BioResources. 2007;2(3):472–499.

13. Bušić A, Marđetko N, Kundas S, Morzak G, Belskaya H, Šantek MI, et al. Bioethanol production from renewable raw materials and its separation and purification: A review. Food Technology and Biotechnology. 2018;56(3):289–311.

14. Galbe M, Wallberg O. Pretreatment for biorefineries: A review of common methods for efficient utilisation of lignocellulosic materials. Biotechnology for Biofuels and Bioproducts. 2019;1(1).

15. Weiss ND, Felby C, Thygesen LG. Enzymatic hydrolysis is limited by biomass-water interactions at high-solids: Improved performance through substrate modifications. Biotechnology for Biofuels and Bioproducts. 2019;12(3).

16. Osipov DO, Bulakhov AG, Korotkova OG, Rozhkova AM, Duplyakin EO, Afonin AV, et al. Effect of the milling of wheat bran on its properties and reactivity during biocatalytic conversion. Catalysis in Industry. 2017;9(1):77–84.

17. Amezcua-Allieri MA, Durán TS, Aburto J. Study of chemical and enzymatic hydrolysis of cellulosic material to obtain fermentable sugars. Journal of Chemistry. 2017;2017.

18. Junejo SA, Geng H, Wang N, Wang H, Ding Y, Zhou Y, et al. Effects of particle size on physicochemical and in vitro digestion properties of durum wheat bran. International Journal of Food Science and Technology. 2019;54(1):221–230.

19. Onipe OO, Beswa D, Jideani AIO. Effect of size reduction on colour, hydration and rheological properties of wheat bran. Food Science and Technology International. 2017;37(3):389–396.

20. Pogorelova NA, Gavrilova NB, Rogachev EA, Schetinina EM. Determining the effectiveness of wheat bran conversion methods for use in food technology. Storage and Processing of Farm Products. 2020;(1):48–57. (In Russ.).

21. Lomovsky OI, Lomovskiy IO, Orlov DV. Mechanochemical solid acid/base reactions for obtaining biologically active preparations and extracting plant materials. Green Chemistry Letters and Reviews. 2017;10(4):171–185.

22. Barbosa FC, Silvello MA, Goldbeck R. Cellulase and oxidative enzymes: New approaches, challenges and perspectives on cellulose degradation for bioethanol production. Biotechnology Letters. 2020;42(6):875–884.

23. Gil-Montenegro AA, Arocha-Morales JS, Rojas-Pérez LC, Narváez-Rincón PC. Process simulation for xylitol production from brewer’s spent grain in a Colombian biorefinery. Part 1: Xylose production from arabinoxilans extracted by the alkaline pretreatment of BSG. Ingeniería e Investigación. 2019;39(1):15–23.

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