Shiraz, Iran
Bushehr, Iran
Bushehr, Iran
Shiraz, Iran
Shiraz, Iran
Kashk is a perishable fermented dairy product. Since chemical preservatives are harmful for human health, we aimed to study lemongrass (Cymbopogon citratus L.) as a natural preservative. First, we assessed the phytochemical properties of lemongrass extract. Then, we added lemongrass extract and microencapsulated lemongrass extract to kashk samples. Finally, we analyzed their physicochemical and sensorial properties during 60 days of storage. Catechin (419.04 ± 0.07 mg/L), gallic acid (319.67 ± 0.03 mg/L), and chloregenic acid (4.190 ± 0.002 mg/L) were found to be the predominant phenolic constituents in lemongrass. Total phenolics, total flavonoids, and antioxidant activity (IC50) values of the lemongrass extract were 26.73 mg GA/g, 8.06 mg Quercetin/g, and 2751.331 mg/L, respectively. The beads were spherical in shape with a 35.03-nm average particle diameter and 47.81% microencapsulation efficiency. The pH of the supplemented kashks decreased during the storage time. They showed lower acid degree values than the control at the end of storage. The peroxide, p-anisidine, and thiobarbituric acid values of the sample fortified with microencapsulated lemongrass extract were 6.15, 4.76, and 44.12%, respectively, being the lowest among the samples. This kashk sample had the highest hardness (570.62 ± 21.87 g), adhesiveness (18.10 ± 4.36 mJ), and cohesiveness (0.56 ± 0.25) but the lowest chewiness (72.66 ± 3.08 mJ) among the samples. It also had a better sensory profile than the control samples. Our results indicated that microencapsulated lemongrass extract could be incorporated into kashk to ensure suitable sensorial and textural properties. Furthermore, it may delay fat oxidation and lipolysis during storage.
Polyphenols, lemongrass extract, encapsulation, fat oxidation, kashk
1. Pourjoula M, Picariello G, Garro G, D'Auria G, Nitride C, Rheza Ghaisari A, et al. The protein and peptide fractions of kashk, a traditional Middle East fermented dairy product. Food Research International. 2020;132. https://doi.org/10.1016/j.foodres.2020.109107
2. Hematian A, Nouri M, Dolatabad SS. Kashk with caper (Capparis spinosa L.) extract: quality during storage. Foods and Raw Materials. 2020;8(2):402-410. https://doi.org/10.21603/2308-4057-2020-2-402-410
3. Lu M, Wang NS. Spoilage of milk and dairy products. In: Bevilacqua A, Corbo MR, Sinigaglia M, editors. The microbiological quality of food. Foodborne spoilers. Woodhead Publishing; 2017. pp. 151-178. https://doi.org/10.1016/B978-0-08-100502-6.00010-8
4. Bensid A, El Abed N, Houicher A, Regenstein JM, Özogul F. Antioxidant and antimicrobial preservatives: Properties, mechanism of action and applications in food - a review. Critical Reviews in Food Science and Nutrition. 2022;62(11):2985-3001. https://doi.org/10.1080/10408398.2020.1862046
5. Golestan L, Seyedyousefi L, Kaboosi H, Safari H. Effect of Mentha spicata L. and Mentha aquatica L. essential oils on the microbiological properties of fermented dairy product, kashk. International Journal of Food Science and Technology. 2016;51(3):581-587. https://doi.org/10.1111/ijfs.13014
6. Haque ANMA, Remadevi R, Naebe M. Lemongrass (Cymbopogon): A review on its structure, properties, applications and recent developments. Cellulose. 2018;25(10):5455-5477. https://doi.org/10.1007/s10570-018-1965-2
7. Kamaruddin ZH, Jumaidin R, Selamat MZ, Ilyas RA. Characteristics and properties of Lemongrass (Cymbopogan citratus): A comprehensive review. Journal of Natural Fibers. 2022;19(14):8101-8118. https://doi.org/10.1080/15440478.2021.1958439
8. Tayel AA, Hussein H, Sorour NM, El-Tras WF. Foodborne pathogens prevention and sensory attributes enhancement in processed cheese via flavoring with plant extracts. Journal of Food Science. 2015;80(12):M2886-M2891. https://doi.org/10.1111/1750-3841.13138
9. Melo AM, Turola Barbi RC, Souza WFC, Luna LC, de Souza HJB, Lucena GL, et al. Microencapsulated lemongrass (Cymbopogon flexuosus) essential oil: A new source of natural additive applied to Coalho cheese. Journal of Food Processing and Preservation. 2020;44(10). https://doi.org/10.1111/jfpp.14783
10. Zhu Q, Pan Y, Jia X, Li J, Zhang M, Yin L. Review on the stability mechanism and application of water-in-oil emulsions encapsulating various additives. Comprehensive Reviews in Food Science and Food Safety. 2019;18(6):1660-1675. https://doi.org/10.1111/1541-4337.12482
11. Felix PHC, Birchal VS, Botrel DA, Marques GR, Borges SV. Physicochemical and thermal stability of microcapsules of cinnamon essential oil by spray drying. Journal of Food Processing and Preservation. 2017;41(3). https://doi.org/10.1111/jfpp.12919
12. Salama HH, El-Sayed HS, Kholif AMM, Edris AE. Essential oils nanoemulsion for the flavoring of functional stirred yogurt: Manufacturing, physicochemical, microbiological, and sensorial investigation. Journal of the Saudi Society of Agricultural Sciences. 2022;21(6):372-382. https://doi.org/10.1016/j.jssas.2021.10.001
13. Bahmanzadegan A, Rowshan V, Zareiyan F, Hatami A. Lagoecia cuminoides L., its antioxidant activity and polyphenolic constituents from Iran. Natural Product Research. 2019;33(16):2376-2378. https://doi.org/10.1080/14786419.2018.1440232
14. Karimi M, Sekhavatizadeh SS, Hosseinzadeh S. Milk dessert containing Lactobacillus reuteri (ATCC 23272) encapsulated with sodium alginate, Ferula assa-foetida and Zedo (Amygdalus scoparia) gum as three layers of wall materials. Food and Bioproducts Processing. 2021;127:244-254. https://doi.org/10.1016/j.fbp.2021.03.003
15. Borhanpour F, Sekhavatizadeh SS, Hosseinzadeh S, Hasanzadeh M, Golmakani M-T, Moharreri M. Effect of microencapsulated chavil (Ferulago angulata) extract on physicochemical, microbiological, textural and sensorial properties of UF-feta-type cheese during storage time. International Journal of Food Engineering. 2022;18(1):53-66. https://doi.org/10.1515/ijfe-2021-0198
16. Siddique A, Park YW. Evaluation of correlation between acid degree value and peroxide value in lipolysis of control and iron fortified caprine milk cheeses during 4 months storage. Open Journal of Animal Sciences. 2018;9(1). https://doi.org/10.4236/ojas.2019.91001
17. Ghendov-Moşanu A, Sturza R, Opriş O, Lung I, Popescu L, Popovici V, et al. Effect of lipophilic sea buckthorn extract on cream cheese properties. Journal of Food Science and Technology. 2020;57(2):628-637. https://doi.org/10.1007/s13197-019-04094-w
18. Gazwi HSS. Preventive effect of lemongrass (Cymbopogon citratus) against oxidation in soybean oil. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences. 2020;90(1):151-159. https://doi.org/10.1007/s40011-019-01091-z
19. Kabtni S, Sdouga D, Bettaib Rebey I, Save M, Trifi-Farah N, Fauconnier M-L, et al. Influence of climate variation on phenolic composition and antioxidant capacity of Medicago minima populations. Scientific Reports. 2020;10(1). https://doi.org/10.1038/s41598-020-65160-4
20. Lahlou Y, Rhandour Z, El Amraoui B, Bamhaoud T. Screening of antioxidant activity and the total polyphenolic contents of six medicinal Moroccan's plants extracts. Journal of Materials and Environmental Sciences. 2019;10(12):1332-1348.
21. Costa G, Grangeia H, Figueirinha A, Figueiredo IV, Batista MT. Influence of harvest date and material quality on polyphenolic content and antioxidant activity of Cymbopogon citratus infusion. Industrial Crops and Products. 2016;83:738-745. https://doi.org/10.1016/j.indcrop.2015.12.008
22. Norcino LB, Mendes JF, Figueiredo JA, Oliveira NL, Botrel DA, Mattoso LHC. Development of alginate/pectin microcapsules by a dual process combining emulsification and ultrasonic gelation for encapsulation and controlled release of anthocyanins from grapes (Vitis labrusca L.). Food Chemistry. 2022;391. https://doi.org/10.1016/j.foodchem.2022.133256
23. Gholamian S, Nourani M, Bakhshi N. Formation and characterization of calcium alginate hydrogel beads filled with cumin seeds essential oil. Food Chemistry. 2021;338. https://doi.org/10.1016/j.foodchem.2020.128143
24. Mehran M, Masoum S, Memarzadeh M. Improvement of thermal stability and antioxidant activity of anthocyanins of Echium amoenum petal using maltodextrin/modified starch combination as wall material. International Journal of Biological Macromolecules. 2020;148:768-776. https://doi.org/10.1016/j.ijbiomac.2020.01.197
25. Zhang S, Li D, Zhang X, Sun Y, Xu S, Wang X, et al. Global transcriptomic analysis of Lactobacillus delbrueckii subsp. bulgaricus ATCC11842 reveals the role of LDB_RS05285 in the post-acidification of yogurt. Food and Function. 2021;12(19):9077-9086. https://doi.org/10.1039/D1FO01357B
26. Kim YK, Nam MS, Bae HC. Characteristics of Gouda cheese supplemented with chili pepper extract microcapsules. Korean Journal for Food Science of Animal Resources. 2017;37(6):833-839. https://doi.org/10.5851/kosfa.2017.37.6.833
27. Suzuki-Iwashima A, Matsuura H, Iwasawa A, Shiota M. Metabolomics analyses of the combined effects of lactic acid bacteria and Penicillium camemberti on the generation of volatile compounds in model mold-surface-ripened cheeses. Journal of Bioscience and Bioengineering. 2020;129(3):333-347. https://doi.org/10.1016/j.jbiosc.2019.09.005
28. Khan IT, Nadeem M, Imran M, Ajmal M, Ali S. Antioxidant activity, fatty acids characterization and oxidative stability of Gouda cheese fortified with mango (Mangifera indica L.) kernel fat. Journal of Food Science and Technology. 2018;55(3):992-1002. https://doi.org/10.1007/s13197-017-3012-y
29. El-Messery TM, El-Said MM, Demircan E, Ozçelik B. Microencapsulation of natural polyphenolic compounds extracted from apple peel and its application in yoghurt. Acta Scientiarum Polonorum, Technologia Alimentaria. 2019;18(1):25-34. https://doi.org/10.17306/J.AFS.2019.0597
30. Pérez-Soto E, Cenobio-Galindo AJ, Espino-Manzano SO, Franco-Fernández MJ, Ludeña-Urquizo FE, Jiménez-Alvarado R, et al. The addition of microencapsulated or nanoemulsified bioactive compounds influences the antioxidant and antimicrobial activities of a fresh cheese. Molecules. 2021;26(8). https://doi.org/10.3390/molecules26082170
31. Siyar Z, Motamedzadegan A, Mohammadzadeh Milani J, Rashidinejad A. The effect of the liposomal encapsulated saffron extract on the physicochemical properties of a functional ricotta cheese. Molecules. 2021;27(1). https://doi.org/10.3390/molecules27010120
32. Gutiérrez G, Matos M, Barrero P, Pando D, Iglesias O, Pazos C. Iron-entrapped niosomes and their potential application for yogurt fortification. LWT. 2016;74:550-556. https://doi.org/10.1016/j.lwt.2016.08.025
33. Mohamed Ahmed IA, Alqah HAS, Saleh A, Al-Juhaimi FY, Babiker EE, Ghafoor K, et al. Physicochemical quality attributes and antioxidant properties of set-type yogurt fortified with argel (Solenostemma argel Hayne) leaf extract. LWT. 2021;137. https://doi.org/10.1016/j.lwt.2020.110389
34. Sah BNP, Vasiljevic T, McKechnie S, Donkor ON. Physicochemical, textural and rheological properties of probiotic yogurt fortified with fibre-rich pineapple peel powder during refrigerated storage. LWT. 2016;65:978-986. https://doi.org/10.1016/j.lwt.2015.09.027
35. Ojagh SM, Hasani S. Characteristics and oxidative stability of fish oil nano-liposomes and its application in functional bread. Journal of Food Measurement and Characterization. 2018;12(2):1084-1092. https://doi.org/10.1007/s11694-018-9724-5
36. Sawale PD, Singh RRB, Arora S. Stability and quality of herb (Pueraria Tuberosa)-milk model system. Journal of Food Science and Technology. 2015;52(2):1089-1095. https://doi.org/10.1007/s13197-013-1067-y
37. Balabanova T, Petkova N, Ivanova M, Panayotov N. Design of Labneh cheese fortified with alginate-encapsulated pepper (Capsicum annuum) extracts. Emirates Journal of Food and Agriculture. 2020;32(8):559-566. https://doi.org/10.9755/ejfa.2020.v32.i8.2132
38. Gorji EG, Waheed A, Ludwig R, Toca-Herrera JL, Schleining G, Gorji SG. Complex coacervation of milk proteins with sodium alginate. Journal of Agricultural and Food Chemistry. 2018;66(12):3210-3220. https://doi.org/10.1021/acs.jafc.7b03915
39. Silva W, Torres-Gatica MF, Oyarzun-Ampuero F, Silva-Weiss A, Robert P, Cofrades S, et al. Double emulsions as potential fat replacers with gallic acid and quercetin nanoemulsions in the aqueous phases. Food Chemistry. 2018;253:71-78. https://doi.org/10.1016/j.foodchem.2018.01.128
40. Ribeiro AM, Estevinho BN, Rocha F. Spray drying encapsulation of elderberry extract and evaluating the release and stability of phenolic compounds in encapsulated powders. Food and Bioprocess Technology. 2019;12(8):1381-1394. https://doi.org/10.1007/s11947-019-02304-z
41. Nishad J, Dutta A, Saha S, Rudra SG, Varghese E, Sharma RR, et al. Ultrasound-assisted development of stable grapefruit peel polyphenolic nano-emulsion: Optimization and application in improving oxidative stability of mustard oil. Food Chemistry. 2021;334. https://doi.org/10.1016/j.foodchem.2020.127561
42. El-Sayed SM, Youssef AM. Potential application of herbs and spices and their effects in functional dairy products. Heliyon. 2019;5(6). https://doi.org/10.1016/j.heliyon.2019.e01989
