PRODUCTION OF BIOETHANOL FROM ROBUSTA COFFEE PULP (COFFEA ROBUSTA L.) IN VIETNAM
Abstract and keywords
Abstract (English):
Coffee pulp is the first waste product obtained during the wet processing of coffee beans. Coffee pulp makes up nearly 40% of the total weight of the coffee cherry. Coffee pulp contains 25.88% of cellulose, 3.6% of hemicel- luloses, and 20.07% of lignin. Coffee pulp is considered as an ideal substrate of lignocellulose biomass for micro- bial fermentation to produce such value-added products as ethanol. In this study, we used alkaline pre-treatment of the coffee pulp with NaOH (0.2 g/g biomass) in a microwave system at 120°C during 20 min. This method gave the best results: 71.25% of cellulose remained, and 46.11% of hemicellulose and 76.63% of lignin were removed. After that, the pre-treated biomass was hydrolyzed by Viscozyme Cassava C (enzyme loading was 19.27 FPU/g) at 50°C for 72 hours. The results showed that the highest reducing sugars and glucose concentration after hydrolysis were 38.21 g/l and 30.36 g/l, respectively. Then, the hydrolysis solution was fermented by S. cerevisiae (3.108 cells/ml) at 30°C for 72 hours. The highest concentration of ethanol obtained was 11.28 g/l. The result illustrated that, available and non- edible as it is, coffee pulp could be a potential feedstock for bioethanol production in Vietnam.

Keywords:
Bioethanol, coffee pulp, Coffea robusta, lignocellulose biomass, hydrolysis, pre-treatment
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Vietnam is currently  the  world’s  largest  exporter of Robusta coffee, as well as the world’s second-larg- est exporter of coffee beans after Brazil. In 2016, the total production of coffee beans in Vietnam was about 1,636,500 tons. About 450,000 tons of dried coffee pulp is produced here annually. Coffee pulp is mainly used as a fuel for fruit/coffee beans drying or as a compost and fertilizer on coffee plantations, which causes serious en- vironmental pollution.

All over the world, there have been many researches on the use of coffee pulp. For instance, feeding and di- gestibility studies were conducted in concrete ponds to evaluate the use of coffee (Coffea robusta) pulp as a par- tial and total replacement for yellow maize in low-cost diets for catfish [1]. The research evaluated the effect of

 

adding coffee husks to animal feed as a substitute for a mixture of corn grain, husks, and cobs. In addition, there have been many studies on how coffee solid wastes can be used. For example, Flammulina velutipes mushroom can be cultivated on coffee spent-ground and  coffee husk [2]. Coffee husk can be used as a carbon source for citric acid production in a solid-state fermentation sys- tem [3] or for wastewater treatment [4]. However, re- searchers are more concerned with producing ethanol from coffee pulp using chemical methods [5]. The prob- lem is that these methods remain limited and eco-un- friendly as coffee pulp hydrolysis requires acid and alkali, which means expensive sophisticated equipment. In addition, coffee pulp has a high concentration of car- bohydrates and, thus, can be used as a potential raw material for bioethanol production [6]. Besides, recent

 

 

Copyright © 2019, Phuong et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material for any purpose, even commercially, provided the original work is properly cited and states its license.

 

 

 

studies indicate that residue utilization has an excellent potential for bioethanol production, given that it does not involve costs related to raw material growth. Further- more, it is estimated that ethanol production from agri- cultural residues could be sixteen times higher than the current production [7].

x

 
Vietnam possesses large quantities of coffee  pulp that need utilization. This is also in line with the cur- rent global trends to seek alternative renewable energy

substances according  to  the  method  offered  by  Chen et al. (2007) [17]. Then the pre-treated residue was pressed to remove excess water and dried at 65°C until moisture content stabilized between 5% and 8%. The concentrations of cellulose, hemicellulose, and lignin remaining in the pre-treated material were calculated by the following equation:

x            p      i

 
R  = A /A × 100,                                (1)

 

sources to replace traditional fossil fuels and solve the

 

where R

 

is the percentage of cellulose (RC), hemicel-

 

problem of environmental pollution and climate change.

Thus, the present study offers a good solution for these

 

lulose (RH), or lignin (RL) remaining in the pre-treated pulp, %; A is the amount of the constituent in the initial

 

problems.

 

dried coffee pulp, g; and A

 

is the amount of the consti-

 

 

i

 

p

STUDY OBJECTS AND METHODS

Materials. Robusta coffee pulp was collected at Pong Drang  commune,  Krong  Buk  district,  Dak  Lak province, Vietnam. The berries were of bright-red co- lour, ripe, neither crushed nor moldy. After harvesting, the pulp was removed and dried at 65°C until the mois- ture content was 5–8%. After that, the pulp was crushed and sieved; the diameter of the powder was 0.5–1 mm. Finally, the powder was packaged in plastic bags and stored under ambient conditions.

Analytical methods. The moisture content was ana- lyzed according to AOAC method 934.06.

The total ash content was determined by using AOAC method 942.05.

The  analysis  of  total  fat  was  performed  by  using


tuent after the pre-treatment of the dried coffee pulp, g.

Hydrolysis method (enzyme loading). 5 mL of Vis- cozyme Cassava C preparation, 150 ml of 0.05 mol/l citrate buffer (pH 4.8), and 15 g (equivalent to 10% of dry material per 100 ml of solution, w/v) of pressed pre-treated dried pulp were mixed in a flask. The con- tainers were incubated in a thermal shaker at 50°C and 150 rpm for 72 hours. After that, the material from each treatment was centrifuged at 2,500 rpm for 10 min [19].

s

The supernatant was removed to determine RSs, total re- ducing sugars (TRS ), and glucose concentrations. The control samples were not treated by heat and alkaline.

The yield from the enzymatic hydrolysis process, %, was calculated using the following equation. Only the cellulose present in the pre-treated coffee pulp was taken into account:

 

AOAC method 948.16.

 

YEH = 0.9(G –G )/C

 

× 100,                    (2)

 

The quantitative analysis of caffeine was per- formed by using a Genesys UV-Vis Spectrometer (Genesis 10S) [8].

The total polyphenol content in the extracts was de- termined according to the Folin-Ciocalteu colorimetric method with some modifications [9].

The micro-Lowry method [10] was used to determine the protein content.

The calcicum pectate method was applied to deter- mine the pectin content [11].

Phenol sulphuric acid was used to estimate the total reducing sugars (TRS) using maltose as standard [12].

The reducing sugars (RS) in the hydrolysate were measured by using the DNS method adapted from Miller [13].

A  Clever  Check  blood  glucose   meter   (model TD 4230, Germany) [14] was used to determine the mo- nomeric sugars (glucose).

The  cellulose,  hemicellulose,  and  lignin  contents

were determined by the crude fibre analysis [15].

The   ethanol   concentration    was    determined with the help of a Genesis UV-Vis  Spectrometer (Genesis 10S) [16].

Pre-treatment method. 50 g of the dried  coffee pulp was treated by 500 ml of sodium hydroxide solu- tion (0.2 g NaOH/g biomass). After that, the mixture

 

e         w         p

 

e

 

w

p

where G is the glucose concentration at the end of the enzymatic hydrolysis, g glucose/l [18]; G is the glucose concentration without enzyme treatment, g  glucose/l; and C is the cellulose concentration in the pre-treated material, g cellulose/l.

4  2         4

2              4                                                            4          2

Fermentation method. After the hydrolysis, the solution was divided into equal portions of 250 ml each and put in an Erlenmeyer flask. Then (NH ) SO (1 g/l), K HPO (0.1 g/l) and MgSO .7H O (0.2 g/l) were ad- ded into the solution. The medium was autoclaved at 121°C for 20 min and cooled at room temperature. Fer- mentation was carried out in an Erlenmeyer flask with

3.108 cells/ml of S. cereviciae at 30°C, 120 rpm, and pH of 5 [20]. The yeast was collected from the Laboratory of the Food Technology Department at the Industrial University of Ho Chi Minh City. Ethanol concentration was analyzed by using a Genesis UV-Vis Spectrometer at different fermentation times:

Y   = EC(G –G ),                               (3)

p/s                      b         e

 

b

e

where EC is ethanol concentration at the end of fermen- tation, g/l; G is glucose concentration at the beginning of the fermentation, g/l; G is glucose concentration at the end of the fermentation, g/l. The percentage of the theoretical ethanol yield was calculated as follows:

 

was pre-treated at 195W and 120°C for 20 min in the microwave system. The pre-treated biomass was reco-

 

 

Y

 

= Y

et            p/s

 

/0.51 × 100,                           (4)

 

vered by filtration and washed with 1,000 mL of hot wa-

ter (70°C) to remove the remaining lignin and alkaline

 

where  0.51  is  the  maximum  theoretical  ethanol  yield

when converting 1g glucose to ethanol.

 

 

 

Table 1. Chemical composition of coffee pulp, g/100g dry basis

 

Table 2. Percentages of R

 

remaining in pre-treated coffee

 

x

 
                                                                                                                pulp

 

Components,

 

Present

 

a           b                c         d         e

 

 %                     study                                                                   

 

Lignocellu-

 

Before pre-treat-

 

After pre-treat-

 

Percentages

 

 

Moisture

content

73.85

77.9

82.0

15.0

losic biomass

ment, g/100g

dry basis

ment, g/100g

dry basis

of R

x

Total sugars

9.18

9.70

 

28.7

Cellulose, %

25.88b

18.44a

71.25

Reducing sugars

8.34

9.63

12.40

 

24.25

Hemicellu- lose, %

3.60b

1.94a

53.89

Starch

10.20

 

Lignin, %

20.07b

4.69a

23.37

 

Pectin                4.37        11.37   6.50                    –

Protein               9.52        10.47   10.1                    –         7.0

Cellulose            25.88      20.7     17.7           23.0   20.6     16.0

 

Hemicellu-

3.60

3.60

2.30

20.0

17.2

11.0

lose Lignin

 

20.07

 

14.30

 

17.5

 

22.0

 

15.5

 

9.0

Lipids

1.22

1.20

 

0.3

Ash

6.29

7.33

8.30

15.4

7.9

5.4

Caffeine             0.78        –          1.3                       –         1.0

 Polyphenols    8.69        –          1.8–8.56              –         5.0     

Note: a[22]; b[23]; c[24]; d [18]; e [25]

 

 

Statistical analysis. All treatments in this study were conducted in triplicate, and 95% of confidence le- vel was applied for the data analysis. ANOVA was used by the one-way analysis of variance, and Statgraphics software (Centurion XV) was used to determine the sta- tistical differences between the treatments.

 

RESULTS AND DISCUSSION

Characteristics of the solid fraction of coffee pulp. Table 1 shows that the cellulose and lignin content in the coffee pulp (Robusta coffea) was 25.88% and 20.07%, re- spectively. These results were higher than those received by Bonilla-Hermosa et al., Elias, and Menezes et al. However, the hemicellulose content was similar with the result obtained by Elias (1979) [18, 22, 23]. These differ- ences can be explained by the fact that the previous stud- ies used Arabica, whereas the present research was based on Robusta coffee.

Coffee husks and pulp are comprised of the outer skin and the attached residual pulp, and these solid re- sidues are obtained after de-hulling of the coffee cher- ries during dry or wet processing, respectively [4]. The coffee pulp only included outer skin and fruit pulp. The sticky coffee husk included skin, fruit pulp, and, perhaps, an insignificant amount of pectin and parchment. There- fore, the total sugars (28.7%) and the reducing sugar content (24.25%) of the sticky coffee husk were higher than those of the coffee pulp (9.7 and 9.63%) [22, 25].

According to Palonen and Hetti [26], lignocellulose biomass is a major structural component of woody plants and other plants, such as grass, rice, and maize. The ma- jor constituents of lignocellulose are cellulose, hemicellu- lose, and lignin. The crude fibre in coffee pulp included: 25.88% of cellulose, 3.6% of hemicelluloses, and 20.07% of lignin. The content of cellulose in the coffee pulp was similar to that in rice husk (24.3%) [27] but lower than in wheat straw (38.2%) [28] and bagasse (38%) [29]. Ho- wever, there was also  a  similar  proportion  between the cellulose content in the coffee pulp (equivalent to

12

 

Note: a and b in the same row denote a significant difference (p < 5%)

 

 

52.23%, g cellulose/100g crude fibre) and the typical proportion of lignocellulose (40–60%) [30]. Therefore, coffee pulp is also considered a source of lignocellulose biomass, which can be used in the production of bioetha- nol (second generation ethanol production).

Alkali pretreatment. According to Sun  and Cheng [31], the pre-treatment process has a number of advantages: it reduces cellulose crystallinity, removes lignin and hemicellulose, and increases the porosity of the materials. Pre-treatment should meet a number of requirements:

  • it cannot produce by-products that are inhibitory to the subsequent hydrolysis and fermentation processes;
  • it cannot result in a loss or degradation of carbohy- drate;
  • it should improve the formation of sugars or the abili- ty to subsequently form sugars by enzymatic hydrolysis; and
  • it has  to  be  cost-effective.  Currently,  pre-treatment of lignocellulosic materials can be chemical, physical, physico-chemical, and biological. As for materials that are rich in lignin, alkaline pre-treatment method seems to be the most efficient one.

The efficiency of pre-treatment depends entirely on the type of alkalis, concentration, time, and tempera- ture of the pre-treatment process. To increase the effi- ciency of lignin removal, the above factors need to be increased. However, the increase in these factors means more cellulose loss. In this study, the coffee pulps were pre-treated with 0.2NaOH/g biomass at 120°C  for 20 min in a microwave system. The results showed th at 71.25% of cellulose was retained, while 46.11% of hemicellulose and 76.63% of lignin were removed. Al- though the result was not high, the conversion efficiency could not be regarded as low.

It was necessary to go through the next stages (hy- drolysis and fermentation) to evaluate the ethanol con- version efficiency. According to [18], when coffee pulp was pre-treated with 4% NaOH (w/v) at 121°C for

25 min, the commercial efficiency removal  of  lignin and hemicellulose was 78,41% and 55.85%, respective- ly, while 69.18% of cellulose was obtained. Wang and Cheng [32] pre-treated coastal Bermuda grass with so- dium  hydroxide  (1%  NaOH)    and  calcium  hydroxide

(0.1 g Ca(OH)2)  (in  g/dry  biomass)  at  121°C  during 30 min and obtained about 75% and less than 20% of lig- nin removal, respectively. In addition, the results of Kim

2

 
and Holtzapple [33] showed that the optimal conditions of pre-treatment for corn stover were 0.5 g Ca(OH) /g

 

 

                     

  1. (b)

Fig. 1. Scanning electron microphotographs (SEM) of coffee pulp. The coffee pulp (a) was not pre-treated and the coffee pulp

  1. ) was pre-treated with 0.2g NaOH/g biomass at 120°C for 25 min.

 

 

raw biomass (55°C). After 4 weeks of pre-treatment, 97.7% of cellulose remained while 32.3% of hemicel- lulose and 66.9% of lignin were removed. This result

 

microwave pre-treatment could significantly increase the cellulose hydrolyzation by enzyme because of a larger contact area of cellulose and enzyme [36]. It also causes

 

2

 
showed that the effect of Ca(OH)

was better than that

 

linkage degradation between cellulose, hemicelluloses,

 

of NaOH because it was cheaper and produced a larger amount of cellulose. However, hydrolysis and fermenta- tion need to be conducted to evaluate its effectiveness mainly because the final product is ethanol.

Fig. 1 shows that NaOH treatment of lignocellulosic materials caused swelling, which increased the internal surface area, decreased the degree of polymerization and crystallinity, separated the structural linkages between lignin and carbohydrates, and disrupted the lignin struc- ture [34]. The mechanism of alkaline hydrolysis is be- lieved to be in the saponification of intermolecular ester bonds which crosslink xylan hemicelluloses and other components, e.g. lignin and other hemicellulose. The po- rosity of the lignocellulosic materials increases with the removal of the crosslinks [35]. In addition, Fig. 1 also shows the difference between the raw and the pre-treated samples. The raw samples were rigid, very compact, and non-porous, while the pre-treated sample showed an in- crease in porosity and a greater surface area, which was attributed to the removal of lignin and hemicellulose.

The alkali pre-treatment can cause  redistribution and solubilization of lignin, as well as an increase in the hydrolyzation of cellulose and the porosity of sub- strates.  However,  alkali  pre-treatment  combined  with

 

Table 3. Concentration of reducing sugars (RS), total reducing sugars (TRS), and glucose after enzymatic hydrolysis

 

Test

TRS, g/l

RS, g/l

Glucose, g/l

References

Coffee pulp (Robusta)

48.2b

38.21b

30.36b

Present study

Control

15.8a

14.15a

12.63a

Present study

Coffee pulp (Arabica)

66.15

38.13

27.02

[18]

Wheat straw

279*

[38]

Bagasse cane

21

[39]

Note: *mg/g biomass; a and b in the same column denote a significant difference (p < 5%)

 

and lignin. In addition, this pre-treatment process has a low temperature and a low alkali concentration, which leads to a lower sugar degradation, and no yeast inhi- bitor agents are released, so ethanol fermentation yield is higher than when no microwave pre-treatment was conducted. This viewpoint corresponds with the re- sults obtained by Zhao et al. [36], who combined alkali pre-treatment and microwave pre-treatment of rice hulls. As a result, the reducing sugar increased by 14% if com- pared with the samples that underwent no microwave pre-treatment. The results obtained by Xu et al. [37] showed that the ethanol yield from fermentation process was nearly 6 times higher than that obtained from the untreated material.

Enzymatic hydrolysis. To assess the effectiveness of alkaline pre-treatment, a certain percentage of hemicel- lulose and lignin was removed. The ability of enzymatic hydrolysis to produce a particular amount of glucose had to be taken into account. In addition, to evaluate the ef- fectiveness of the pre-treatment process, a control sam- ple was established. The control samples (not pre-treated with alkali) were also hydrolyzed simultaneously with the test sample under the same conditions. However, the hydrolysis effect was completely different (Table 3).

Table 3 shows that YEH (yield of enzymatic hydroly- sis) was 76.8% (the result was calculated by formula 2). It means that 76.8% of cellulose was converted to glu- cose after the hydrolysis process. The productivity was relatively high. According to Menezes et al., hydrolysis of coffee pulp (Arabica) resulted in producing 27.02 g/l of glucose and 60.48% of hydrolysis efficiency [18].

Silverstein et al. pre-treated cotton stalks and stems with a NaOH solution (2% NaOH, 60 min, 121°C) and obtained conversion of cellulose equal to 60.8% after the enzymatic hydrolysis. This productivity was lower than the results obtained in the study [40], which can be explained by the following factors. When the alkaline pretreatment of lignocellulose was combined with the

13

 

 

 

Table 4. Glucose and ethanol contents, ethanol yield (Y  )

 

Table 5. Comparison of literature data on ethanol production

 

p/s

                                                                                                                from lignocellulose biomass

 

Fermenta-

 

Glucose,

 

Ethanol,

 

Y  , g et/g

 

Y (ethanol                                                                                                                 

 

tion time, h

 

g glucose/l

 

g et/l

 

p/s

glucose

 

et

yield), %

 

Lignocellulose

 

Ethanol

 

Y  , g et/g

 

References

 

0                      36.58*,f

 

p/s

 biomass                    production               glucose                            

 

 

24

9.54e

7.11b

0.27a

51.56a

Sticky coffee

13.6 g/l

0.38

[25]

48

7.42d

10.74c

0.36c

72.20d

husks

(8.49 g/100g)

 

 

72

6.10c

11.28d

0.37c

72.55d

Corn stalks

5.0 g/l

0.44

[44]

72 (control)

4.17a

5.30a

0.31b

60.70b

Barley straw

10.0 g/l

0.44

[44]

96

5.55b

11.13d

0.35bc

70.30c

Sweet sorghum

16.2 g/l

0.31

[21]

 

Note: *Including the glucose concentration (g/l) in hydrolysis solution and in yeast culture supplemented during the fermentation. Various lowercase letters (a, b, c, d, e, f) in the same column denote a signifi- cant difference (p < 5%)

bagasse

 

 

 

Wheat straw

18.1 g/l

0.32

[21]

Corn stover

16.8 g/l

0.33

[45]

Coffee pulp (Arabica)

11.99 g/l

0.40

[18]

 

microwave hydrolysis, the hydrolysis yield was higher.

 

Coffee husk           7.9 g/l

 

[46]

 

The hydrolyzed sugar content also improved, as com- pared to the case when alkaline pre-treatment was used on its own. In addition, if combined with microwaves,

 

Coffee pulp (Robusta)

 

11.28

(11.36 g/100g)

 

0.37               Present

study

 

hydrolysis of the alkaline pre-treatment increases the surface area of cellulose from the breakdown of the hemicellulose and lignin layers. As a result, the sensi- tivity of hydrolysis enzymes increases [41, 42]. On the other hand, Chen et al. [17] pre-treated  barley  straw with 2% NaOH at 121°C. According to their experi- ment, 74.03–84.89% of cellulose conversed to glucose during the enzymatic hydrolysis process. The cellulase concentration was 40–60 FPU/g cellulose supplemented with cellobiase (Novozyme 188). Their result was bet- ter than that of this study, with an enzyme loading of

19.27 FPU/g of substrate.

The productivity increased considerably when the amount of enzyme was increased (FPU/g), or enzyme cellobiase was supplemented (CBU/g). Chen et al. pre-treated corn  straw  with  a  2%  NaOH  solution  for 1 hour at 80°C with an 8% substrate and 20 FPU cellu- lase/g substrate (which contained 1.64 CBU/g) [43]. The hydrolysis process produced a RS concentration equal to 52 g/l and a high amount of cellobiose. In addition, the RSs content peaked at 64.1 g/l after 60 hour with an in- crease in the activity of cellobiase to 10 CBU/g substrate. The cellulase activity is inhibited by cellobiose and glu- cose (to a lesser extent). These problems can be solved by adding cellobiase. In the hydrolysis of lignocellulo- sic biomass, cellulases attack the cellulose chain to form glucose and cellobiose, then cellobiose decomposes to glucose by cellobiase. Thus, the presence of cellobiase helps reduce the inhibition of cellulase by cellobiose and results in a higher yield of sugars [31].

Fermentation. The glucose concentration in the hy- drolysate was consumed by the yeast. The initial con- centration was 36.58 g/l and decreased to 6.1 g/l after

72 hour of fermentation. The final concentration of ethanol was 11.28 g/l with a yield of 0.37 g ethanol/g glucose. The control sample (unprotected by hydrolysis and fermentation) showed the initial glucose concentra- tion of 20.85 g/l and 4.17 g/l at the end of fermentation. The ethanol concentration was only 5.3 g/l (Table 4). Therefore, it was necessary to conduct a pre-treatment process to remove lignin and hemicellulose.

According to Menezes et al., coffee pulp (Arabica)

was  pre-treated  by  a  4%  NaOH  solution  (w/v)  (equi-

 

valent to 0.2 g/g biomass) and then was hydrolysed with

13.82 FPU/g of cellulase [18]. The  results  showed that 27.02 g of glucose/l and 11.99 g of ethanol/l were achieved after hydrolysis and fermentation (Table 5).

In this study, the pre-treatment process was com- bined with the microwave system. As a result, the hy- drolysis efficiency was high (30.36 g of glucose/l was formed) (Table 3). However, the ethanol yield from fermentation was only 11.28 g/l, which was slightly lo- wer than that of the study by Menezes et al. because of the yeast strain or its amount. Besides, they used 3 g/l of dry yeast while the yeast in this study was used as a secondary breed and then added to the hydrolysate with a cell density of 3.108 (cells/ml). Chen et al. pre-trea- ted barley straw with a 2% NaOH solution at 121°C for 1 hour, and the pre-treated material was hydrolysed by Celluclast 1.5 l at a concentration of 40 FPU/g glucose and Novozyme 188 (cellobiase) [17].  The  hydrolysate was inoculated by S. cerevisiae (ATCC 24859) and in- cubated at 30°C for 72 hour. At the end of the fermenta-

tion, the ethanol yield (Yp/s) was 0.31 g ethanol/g glucose, which is lower than the result of this study (0.37).

The coffee pulp was hydrolysed by using sulphuric acid concentrations of 1%, 2%, and 4% for 1 hour; the

achieved  ethanol  concentrations  were  6.097,   4.395, and 3.323 g/l, respectively [47]. The dilute acid hydro- lysis resulted in a low ethanol production  compared with the deionised water hydrolysis. The maximum ethanol concentration of 6.315 g/l was obtained from the coffee pulp, which was hydrolysed by deionised water [48]. Sugar cane bagasse was pre-treated by steam explosion and hydrolysed by cellulase (26 g RS/l in hy- drolysate).  The  final  concentrations  of  ethanol  were

7.4 g/l (0.28 g ethanol/g RS) and 8.2 g/l (0.31 g ethanol/g RS) when two types of yeast were used, i.e. S. cerevisiae ATCC96581 and S. cerevisiae TMB3001 [39].

150 ml of filtrate yeast (Saccharomyces cerevisiae) was added at a concentration of 5.0 g/l and subjected to fermentation for 48 hour at 30°C in a shaker incubator at 120 rpm. The ethanol yield in the fermented broth was found to be 0.50; 0.46; and 0.46 g/g sugar in squeezed

CAP,  DCP,  and  WCP.  The  theoretical  ethanol  yields

 

 

 

 

(Y

 
max

%) of squeezed cashew apple pulp, dry coffee pulp,

 

fermentation broth was high. The high glucose content

 

and wet coffee pulp were found to be 46.0; 9.35; and

40.0%, respectively [5].

Other studies showed that after fermentation barley straw produced the final ethanol concentration equalled to 10 g/l [44]. In addition, Gouvea et al. [25] indicated that when coffee husk was fermented in water (13%, w/v) with S. cerevisiae commercial Baker’s yeast, the fi- nal ethanol concentration equalled 13.6 g/l (Table 5).

The conversion of glucose to  high  or  low  etha- nol content is attributed either to  the  low  concentra- tion of glucose in the hydrolysis solution or to the poor pre-treatment process, or to the low cellulose content in the raw material. This is consistent with the study con- ducted by Belkacemi et al. [44] that showed an ethanol conversion efficiency of 0.44, while the ethanol content was 5 g/l (Table 5). However, Ballesteros et al. obtained quite opposite results [21]: the conversion efficiency reached only 0.32 while the obtained ethanol content was 18.1 g/l (Table 5). Thereby, the conversion efficien- cy of ethanol indicated that the glucose content in the

 

showed that the hydrolysis process or the fermentation

process was very effective.

 

CONCLUSION

One  hundred  gram  of  dry  coffee  pulp  produced

11.36 g of ethanol (the corresponding glucose conversion efficiency of ethanol was 0.37). Comparing with the lite- rature data, it can be seen that ethanol production by fermentation is quite potential. In addition, the present study offers some methods to improve ethanol yields, including the use of a combination of yeast strains for xylose fermentation, hydrolysis coupled with concur- rent fermentation, or selection of more potent strains of

S. cerevisiae.

 

ACKNOWLEDGMENTS

The authors would like to thank the Institute of Bio- technology and Food Technology, the Industrial Univer- sity of Ho Chi Minh City (Vietnam), and our colleagues for the technical and equipment support.

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