The screening of 8 probiotic strains (Propionibacterium, Lactobacillus and Bifidobacterium) characterized by high cholesterol-lowering activity was carried out in the scientific research laboratory of East Siberia University of Technology and Management (Ulan-Ude, Russia). In order to research biotechnological potential of probiotic microorganisms and to conduct statistical analysis we used a mix of standard and modern physicochemical, biochemical and microbiological methods. The strains Lactobacillus helveticus 35-1, Propionibacterium shermanii AC-2503 and Bifidobacterium longum DK-100 were found out to have the highest cholesterol-lowering properties. It can be seen that cocultivation of Lactobacillus helveticus 35-1 and Propionibacterium shermanii AC-2503 strains shows high population density, cholesterol-metabolizing, antimutagenic, adhesive properties and exopolysaccharide biosynthesis. It indicates stable symbiotic bonds between cultures, which served as the basis for making a combined ferment. The analysis of biotechnological potential of Lactobacillus helveticus 35-1 and Propionibacterium shermanii AC-2503 strains and the study of organoleptic, physicochemical, probiotic properties and cholesterol metabolizing activity of the combined ferment have allowed to establish an optimum culture ratio in the ferment - 5 : 95. Optimum cultivation conditions of the continuous culture Lactobacillus helveticus 35-1 and the combined ferment were selected, process parameter of biologically active supplement and frozen ferments of direct loading were substantiated.
bifidobacteria, propionic bacteria, lactobacteria, cholesterol-lowering activity, exopolysaccharide, bacterial concentrate
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