Abstract and keywords
Abstract (English):
Investigation of the composition and microstructure of dietary fiber derived from plants showed that the stabilizers investigated differ with regard to size and shape of the particles and the density of particle distribution. The composition and microstructure of dietary fiber derived from plants have been studied using electron microscopy. Spectrometric profiles of chemical composition have been obtained, and the content of the predominant chemical elements in food microstructure stabilizers has been determined. Some similarity concerning the content of certain chemical elements and the ratio of the contents of different elements has been detected upon the analysis of food structure stabilizers of the same type (carboxymethylcellulose, gum, and sodium pyrophosphate). Mathematical processing of photomicrographs of structure stabilizer samples has been performed, and masks for the assessment of the content of microcavities in the particles of the structure stabilizers investigated have been created.

dietary fiber, stabilizer, microstructure, electron microscopy, histogram, carboxymethylcellulose, sodium alginate, sodium pyrophosphate, xanthan gum


Dairy products are among the most important components of human food. They account for 20% of protein supply and 30% of fat supply in the human diet. Creation of products with predefined properties and rational use of raw materials are the priority directions for the development of dairy products manufacturing technologies [1, 5, 6].
Adherence to scientifically based formulations and compliance of the final product composition with regulatory requirements concerning the composition of raw materials are among the most important issues to be controlled during the assessment of quality of dairy products. Hundreds of dairy products available on the market are in constant demand and often actively marketed; therefore sellers and manufacturers of dairy products alike are always tempted to adulterate these products. Therefore, reliable methods for the identification of raw materials found in dairy products are necessary to prevent faulty and adulterated foods from being sold [2, 7, 8, 9].

The question of reliable determination of the type of components found in dairy products is currently especially acute due to the widespread adulteration of foods with texture stabilizers. The use of these components implies adding them to foods to induce gelling of liquid systems. Structure stabilizers currently in use comprise anionic polysaccharides, both natural (pectin, agar, agaroid, and pyrophosphate) and artificial (oxidized starch). Alginates, cellulose derivatives, and carboxymethyl cellulose (CMC), as well as various gums, are widely used abroad [3, 10, 11, 12]. Classification of stabilizers can be based on one of the following criteria: description of all compounds as polysaccharide materials, assignment of names referring to botanical species, origin (plant, animal, or artificial), or chemical properties. Classification taking the origin of the stabilizers into account is currently preferred; according to this classification, all stabilizers are assigned to groups of modified natural or semi-synthetic stabilizers, chemically modified natural stabilizers or compounds similar to them, or synthetic gums obtained by chemical synthesis.

Agar is one of the classic structure stabilizers widely used in confectionery industry. However, the increasing shortage of agar sources necessitates the replacement of agar with other structure stabilizers. Various types of pectins are an example of promising structure stabilizers. They are currently used in food and pharmaceutical industry. Pectins are capable of forming gel systems characterized by a specific set of physical and chemical properties. Furthermore, pectin was shown to exert beneficial effects on the human organism, and the resources for pectin production are virtually unlimited [4, 13, 14, 15].

The aim of the present work was to compare the microstructure and composition of various structure stabilizers of plant origin for the subsequent development of procedures for the detection of adulterated products.


1. Anisenko, O.V., Stepavenko, O.V., and Shcherbatenko, A.V. Sposoby immobilizatsii ureazy na neorganicheskikh nositelyakh (Methods of urease immobilization on inorganic carriers), Biologiya - nauka XXI veka: trudy VIII Mezhdunarodnoy Pushchinskoy shkoly-konferentsii molodykh uchenykh (Pushchino, 17-21 maya 2001 g.) (Biology in the XXI century: Proceedings of the VIII International Pushchino School and Conference for Young Researchers (Pushchino, May 17-21, 2001)), Pushchino, 2004, p. 249.

2. Beregova, I.V., Pektiny i karraginany v molochnykh produktakh novogo pokoleniya (Pectins and carrageenans in dairy products of the new generation), Molochnaya promyshlennost´ (Dairy industry), 2006, no. 6, pp. 44-46.

3. Dankvert, S.A. and Dukin, I.M., Sovremennoe sostoyanie i perspektivy razvitiya molochnogo kompleksa Rossii (Current state and perspectives of development of the dairy industry in Russia), Molochnaya promyshlennost´ (Dairy industry), 2006, no. 1, pp. 10-11.

4. Kitova, A. E., Kuvichkina, T.N., Arinbasarova, A. Yu., et al., Degradatsiya 2,4-dinitrofenola svobodnymi i immobilizovannymi kletkami Rhodococcus erythropolis HL PM-1 (Degradation of 2,4-dinitrophenol by free and immobilized cells of Rhodococcus erythropolis HL PM-1), Prikladnaya biokhimiya i mikrobiologiya (Applied Biochemistry and Microbiology), 2004, vol. 40, no. 3, pp. 307-311.

5. Makeeva, I.A., Nauchnyye podkhody k formirovaniyu ponyatii potrebitel´skikh svoystv i kharakteristik molochnykh produktov v period intensivnogo razvitiya ikh assortimenta (Scientific approaches to the formation of concepts of consumer properties and characteristics of dairy products during intensive expansion of the range thereof), Khranenie i pererabotka sel´khozsyr´ya (Storage and processing of agricultural raw materials), 2006, no. 3, pp. 48-53.

6. Ashin, V.V., RF Patent 2 007 144 734, 2009.

7. Samuilova, O.K. and Vladimova, L.Ya., Funktsii stabilizatorov i emul´gatorov v molochnykh produktakh (Functions of stabilizers and emulsifiers in dairy products), Pererabotka moloka (Milk processing), 2004, no. 2, p. 22.

8. Cheng, J., Teply, B.A., Sherifi, I., et al., Formulation of functionalized PLGA-PEG nanoparticles for in vivo targeted drug delivery, Biomaterials, 2007, vol. 28, pp. 869-876.

9. Sharma, A., Qiang, Y. Antony, J. et al., Dramatic increase in stability and longevity of enzymes attached to monodispersive iron nanoparticles, IEEE Trans. Magn., 2007, vol. 43, pp. 2418-2420.

10. Gu, H., Xu, K., and Xu, C., Biofunctional magnetic nanoparticles for protein separation and pathogen detection, J. of the American Chemical Society Chem. Commun., 2006, pp. 941-949.

11. Jeng, J., Lin, M.F., and Cheng, F.Y., Using high-concentration trypsin-immobilized magnetic nanoparticles for rapid in situ protein digestion at elevated temperature, Rapid Commun. Mass Spectrom., 2007, vol. 21, pp. 3060-3068.

12. Kaushik, A., Khan, R., Solanki, P.R., et al., Iron oxide nanoparticles-chitosan composite based glucose biosensor, Biosens. Bioelectron., 2008, no. 24, pp. 676-683.

13. Lee, J., Lee, Y., Youn, J.K. et al, Simple synthesis of functionalized superparamagnetic magnetite/silica core/shell nanoparticles and their application as magnetically separable high-performance biocatalysts, Small, 2008, no. 4, pp. 143-152.

14. Kim, M.J., An, G.H., and Choa, Y.H., Functionalization of magnetite nanoparticles for protein immobilization, Diffus. Defect Data, Pt. B, 2007, pp. 895-898.

15. Qiu, J., Peng, H., and Liang, R., Ferrocene modified Fe3O4-SiO2 magnetic nanoparticles as building blocks for construction of reagentless enzyme-based biosensors, Electrochem. Comm., 2007, no. 9, pp. 2734-2738.

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